While there is trial data for each of the vaccines in use, there are limited data regarding the quantitative changes in antibody concentrations over time following vaccination. Graphpad prism 4 parameter logistic curve fit trial# With the introduction of a national immunization program, it will be important to understand the antibody response to immunization in terms of development, peak concentration, and decline over time to assess efficacy of the vaccination delivery. To do this, two elements are required: a robust quantitative SARS-CoV-2 IgG method, which is directed against the spike protein, and a commutable standard or reference material to allow comparison of results across different methods and thus different trials or immunization programs ( 5). This study evaluated several assay performance criteria (such as precision and sensitivity) using recognized and standardized evaluation protocols (EPs) for the new Abbott SARS-CoV-2 IgG II Quant method on the Alinity i system (Abbott Diagnostics, Chicago, IL, USA) and explored the different materials available that may form the basis of a candidate international reference standard for harmonization programs ( 6).Īs the pandemic progresses, new variants of the virus emerge, raising concern that the mutations in these variants may render immunoassays ineffective, as the antigenic changes that arise may no longer represent the antigenic regions of the reagents in the assay. The main SARS CoV-2 lineage circulating in the autumn of 2020 in the United Kingdom was B.1.177 (the Spanish lineage). However, since January 2021, the “UK variant” (VOC 202012/V1 or B1.1.7) has become the predominant virus in the United Kingdom ( 9).
Graphpad prism 4 parameter logistic curve fit trial#įurthermore, by the beginning of March 2021, there have been in total 266 confirmed and probable cases of a “South Africa” variant (VOC 202012/V2 (B.1.351).Graphpad prism 4 parameter logistic curve fit verification#.
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